Journal: Cells

Article Title: BK Ca ( Slo ) Channel Regulates Mitochondrial Function and Lifespan in Drosophila melanogaster

doi: 10.3390/cells8090945

Figure Lengend Snippet: slo 1 mutants reveal accelerated aging. Drosophila BK Ca ( slo 1 ) mutants show significantly reduced lifespan of females ( A ) and males ( B ) by approximately 50% compared to wild-type (wt) flies. The inset shows 50% survival for wt (black) and slo 1 (gray), which was reduced significantly for slo 1 mutants. ( C ) Negative geotaxis assay for wt and slo flies at young (day 3) and older flies (day 30) shows reduced ability of slo 1 mutants to climb the marked distance in a given time in vials compared to their controls. ( D ) Increased polyubiquitination staining is observed in slo 1 mutants (red) as compared to wt in both young and older ages and quantification is provided in ( E ). ( F ) slo 1 mutants show increased intestinal perforations as determined by the leakage of fluorescein dye (green) from the gut unlike control flies, which only show the dye in their gut at both young and older ages. ( G ) Quantification of fluorescein signal from ( F ). ( H ) Microarray data showing differential expression of life span-related genes in wt and slo 1 mutants.

Article Snippet: Flies were fed fluorescein (2% ( w/v ) in media) dye for 9 h and imaged under a fluorescent microscope using 10X objective (Zeiss) (n = 5 independent experiments).

Techniques: Staining, Control, Microarray, Quantitative Proteomics

Journal: Scientific Reports

Article Title: The Role of AMPARs in the Maturation and Integration of Caudal Ganglionic Eminence-Derived Interneurons into Developing Hippocampal Microcircuits

doi: 10.1038/s41598-019-41920-9

Figure Lengend Snippet: The density of dendritic but not somatic glutamatergic synapses is determined by the presence or absence of GluA2. ( A , B ) Images show (from two different angles) Airyscan (LSM) super-resolution images of green fluorescent puncta (PSD-95_EGFP) detected on magenta fluorescent (tdTomato) dendrites and somata converted using Imaris software (Bitplane). PSD-95_EGFP expression that created a punctate signal on the dendrites and soma, is used as a proxy for glutamatergic synaptic sites in 3D space. The tdTomato signal was used to create a surface for dendrites and soma and the EGFP signal that did not fall within 1 µm distance from the created surface was first automatically and then, if necessary, manually eliminated from the analysis. Scale bars, 3 µm. small panels to the right represent the raw images used for analysis. ( C ) Bar graphs of the density of synaptic sites on dendrites (WT, n = 64; GluA2 KO, n = 30; GluA1-3 KO, n = 34) or soma (WT, n = 27; GluA2 KO, n = 22; GluA1-3 KO, n = 13) of the CGE interneurons. The data for all three groups were compared for significance with ANOVA and a posthoc Wilcoxon-Mann-Whitney Test when a significance was found with ANOVA. The density of synaptic sites on soma are similar in all groups compared. The density of synaptic sites is significantly reduced on KO CGE interneurons compared to WT: GluA2 KO to WT, p = 0.034 × 10 −8 , GluA1-3 KO to WT, p = 0.024 × 10 −8 , GluA1-3 to GluA2, p = 0.53. ***p < 0.0005.

Article Snippet: Fluorescent images were captured using the 10X objective of a Zeiss LSM fluorescent microscope and 10 images of hippocampi from each mouse were used to count.

Techniques: Software, Expressing, MANN-WHITNEY